Hotspot

psbT-psbN

LSC · 74421-74484 · 64 bp

Back to Tipularia

Pi 0.0635

MarkerSeek score 74.70

Primer available yes

Label psbT-psbN

Feature Metadata

label_name: psbT-psbN
feature_type: igs
region: LSC
length_bp: 64
variable_sites: 6
pi: 0.0635
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 74.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbT-psbN_p1	CGAGAACCACCTAAAGTTCCA	TCGAAGAACATGGGGACTAG	47.6	50.0	57.6	56.4	122-123 bp	1.0	60.3
psbT-psbN_p2	GCGATCGAATCTATGGAAGCA	TCGAAGAACATGGGGACTAG	47.6	50.0	58.3	56.4	203-204 bp	1.0	59.0
psbT-psbN_p3	CGAGAACCACCTAAAGTTCCA	TTCGAAGAACATGGGGACTAG	47.6	47.6	57.6	57.1	123-124 bp	1.0	58.3
psbT-psbN_p4	GCGATCGAATCTATGGAAGC	TCGAAGAACATGGGGACTAG	50.0	50.0	56.6	56.4	203-204 bp	1.0	58.2
psbT-psbN_p5	GCGATCGAATCTATGGAAGCA	TTCGAAGAACATGGGGACTAG	47.6	47.6	58.3	57.1	204-205 bp	1.0	57.1