Hotspot

trnT-UGU-trnL-UAA

LSC · 46455-47025 · 571 bp

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Pi 0.0292
MarkerSeek score 73.20
Primer available yes
Label trnT-UGU-trnL-UAA

Feature Metadata

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-UGU-trnL-UAA_p1 AGAAAAAGAGCCGGCTATCG ACCAATTTCGCCATATCCCC 50.0 50.0 58.1 58.0 661-672 bp 1.0 86.2
trnT-UGU-trnL-UAA_p2 GCGATGCTCTAACCTCTGAG ACCAATTTCGCCATATCCCC 55.0 50.0 57.9 58.0 610-621 bp 1.0 85.9
trnT-UGU-trnL-UAA_p3 AGAAAAAGAGCCGGCTATCG TAGCGTCTACCAATTTCGCC 50.0 50.0 58.1 58.1 669-680 bp 1.0 85.7
trnT-UGU-trnL-UAA_p4 GCGATGCTCTAACCTCTGAG TAGCGTCTACCAATTTCGCC 55.0 50.0 57.9 58.1 618-629 bp 1.0 85.3
trnT-UGU-trnL-UAA_p5 GGAATCGAACCGATGACCAT ACCAATTTCGCCATATCCCC 50.0 50.0 57.8 58.0 642-653 bp 1.0 84.9