Hotspot

psbB

LSC · 60483-62009 · 1527 bp

Back to Tinospora

Pi 0.445

MarkerSeek score 71.80

Primer available yes

Label psbB

Feature Metadata

label_name: psbB
feature_type: gene
region: LSC
length_bp: 1527
variable_sites: 297
pi: 0.445
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.44
markerseek_score: 71.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbB_p1	TCCCTCAATCCTTCTTGGGT	ACCGCTTGTGGAACTTCAAT	50.0	45.0	57.9	58.0	1297-2139 bp	1.0	91.4
psbB_p2	TTGGGCAAAAGCAGACGTAT	ACCGCTTGTGGAACTTCAAT	45.0	45.0	58.1	58.0	1756-2593 bp	1.0	91.1
psbB_p3	TCCCTCAATCCTTCTTGGGT	AAATGGCAATTGGCGCATAC	50.0	45.0	57.9	58.1	1258-2100 bp	1.0	91.0
psbB_p4	TCCCTCAATCCTTCTTGGGT	CTGACCAGGAACAGGACCTA	50.0	55.0	57.9	58.1	1544-2386 bp	1.0	91.0
psbB_p5	TTGGGCAAAAGCAGACGTAT	AAATGGCAATTGGCGCATAC	45.0	45.0	58.1	58.1	1717-2554 bp	1.0	90.7