Hotspot

ndhF-rpl32

SSC · 110253-110955 · 703 bp

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Pi 0.0343
MarkerSeek score 73.40
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
703
variable_sites
24
pi
0.0343
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
73.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 AACAGGAAGTGGAGCGAAAG AGTACGTTTCTTCGGAACCG 50.0 50.0 58.1 57.9 781-1136 bp 1.0 81.0
ndhF-rpl32_p2 GAACAGGAAGTGGAGCGAAA AGTACGTTTCTTCGGAACCG 50.0 50.0 58.1 57.9 782-1137 bp 1.0 80.9
ndhF-rpl32_p3 CCGTCGGAATAAGGAGAAGC AGTACGTTTCTTCGGAACCG 55.0 50.0 58.1 57.9 821-1176 bp 1.0 80.6
ndhF-rpl32_p4 CGTCGGAATAAGGAGAAGCC AGTACGTTTCTTCGGAACCG 55.0 50.0 58.1 57.9 820-1175 bp 1.0 80.6
ndhF-rpl32_p5 GCCGTCGGAATAAGGAGAAG AGTACGTTTCTTCGGAACCG 55.0 50.0 58.1 57.9 822-1177 bp 1.0 80.6