Hotspot

petD-rpoA

LSC · 77347-77534 · 188 bp

Back to Tinospora

Pi 0.027

MarkerSeek score 69.50

Primer available yes

Label petD-rpoA

Feature Metadata

label_name: petD-rpoA
feature_type: igs
region: LSC
length_bp: 188
variable_sites: 5
pi: 0.027
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 69.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD-rpoA_p1	GGTTGGGTATTGGAGCAACA	GCATTTCGTAATGGATTTACCGA	50.0	39.1	58.1	57.6	311-335 bp	1.0	54.0
petD-rpoA_p2	TGGTTGGGTATTGGAGCAAC	GCATTTCGTAATGGATTTACCGA	50.0	39.1	58.1	57.6	312-336 bp	1.0	54.0
petD-rpoA_p3	GGTTGGGTATTGGAGCAACA	AGCATTTCGTAATGGATTTACCG	50.0	39.1	58.1	57.6	312-336 bp	1.0	54.0
petD-rpoA_p4	TGGTTGGGTATTGGAGCAAC	AGCATTTCGTAATGGATTTACCG	50.0	39.1	58.1	57.6	313-337 bp	1.0	54.0
petD-rpoA_p5	GGTTGGGTATTGGAGCAACA	GCATTTCGTAATGGATTTACCG	50.0	40.9	58.1	56.2	311-335 bp	1.0	51.0