Hotspot

trnP-TGG-psaJ

LSC · 66299-66611 · 313 bp

Back to Tinospora

Pi 0.0415

MarkerSeek score 70.40

Primer available yes

Label trnP-TGG-psaJ

Feature Metadata

label_name: trnP-TGG-psaJ
feature_type: igs
region: LSC
length_bp: 313
variable_sites: 13
pi: 0.0415
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-TGG-psaJ_p1	GCCCATAGGAGTAGGTAGGG	ACTTAACACAGGTGCCACAG	60.0	50.0	58.1	58.0	447-915 bp	1.0	80.8
trnP-TGG-psaJ_p2	ACCCGTGACATTTTGTACCC	ACTTAACACAGGTGCCACAG	50.0	50.0	58.1	58.0	413-881 bp	1.0	80.5
trnP-TGG-psaJ_p3	CCCGTGACATTTTGTACCCA	ACTTAACACAGGTGCCACAG	50.0	50.0	58.1	58.0	412-880 bp	1.0	80.5
trnP-TGG-psaJ_p4	ACAGGATTTGAACCCGTGAC	ACTTAACACAGGTGCCACAG	50.0	50.0	58.1	58.0	424-892 bp	1.0	80.5
trnP-TGG-psaJ_p5	CAGGATTTGAACCCGTGACA	ACTTAACACAGGTGCCACAG	50.0	50.0	58.1	58.0	423-891 bp	1.0	80.4