Hotspot

trnF-GAA-ndhJ

LSC · 47671-48406 · 736 bp

Back to Tinospora

Pi 0.0246

MarkerSeek score 71.70

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 736
variable_sites: 18
pi: 0.0246
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 71.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	GAAAGCTGGATAGGATGGCC	50.0	55.0	58.1	58.1	845-883 bp	1.0	80.8
trnF-GAA-ndhJ_p2	GCCCAAGCCCTCTAGTAGTA	GAAAGCTGGATAGGATGGCC	55.0	55.0	57.9	58.1	926-964 bp	1.0	80.4
trnF-GAA-ndhJ_p3	CCTCGTGTCACCAGTTCAAA	ATCATCCACGCTTGAAACGT	50.0	45.0	58.1	58.2	877-915 bp	1.0	80.0
trnF-GAA-ndhJ_p4	GCCCAAGCCCTCTAGTAGTA	ATCATCCACGCTTGAAACGT	55.0	45.0	57.9	58.2	958-996 bp	1.0	79.6
trnF-GAA-ndhJ_p5	CCTCGTGTCACCAGTTCAAA	TTTGATGCCTGAAAGCTGGA	50.0	45.0	58.1	57.7	855-893 bp	1.0	79.3