Hotspot

psbC-trnS-TGA

LSC · 34517-34711 · 195 bp

Back to Tinospora

Pi 0.0568
MarkerSeek score 83.60
Primer available yes
Label psbC-trnS-TGA

Feature Metadata

label_name
psbC-trnS-TGA
feature_type
igs
region
LSC
length_bp
195
variable_sites
10
pi
0.0568
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.90
markerseek_score
83.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbC-trnS-TGA_p1 AGCTGCAGCAGGATTTGAAA GGTTGATAGCTCCGGTCTTG 45.0 55.0 58.4 58.1 330-345 bp 1.0 82.9
psbC-trnS-TGA_p2 GCTGCAGCAGGATTTGAAAA GGTTGATAGCTCCGGTCTTG 45.0 55.0 57.6 58.1 329-344 bp 1.0 82.4
psbC-trnS-TGA_p3 AGCTGCAGCAGGATTTGAAA GATGGCCGAGTGGTTGATAG 45.0 55.0 58.4 58.1 341-356 bp 1.0 82.3
psbC-trnS-TGA_p4 AGCTGCAGCAGGATTTGAAA GGGTTCGAATCCCTCTCTCT 45.0 55.0 58.4 58.2 276-291 bp 1.0 82.1
psbC-trnS-TGA_p5 GCTGCAGCAGGATTTGAAAA GATGGCCGAGTGGTTGATAG 45.0 55.0 57.6 58.1 340-355 bp 1.0 81.7