Hotspot

ycf1

SSC · 11515-17040 · 5526 bp

Back to Tillandsia

Pi 0.0075

MarkerSeek score 62.70

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5526
variable_sites: 670
pi: 0.0075
unique_haplotype_count: 138
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	CCCTTGTCTCTTCCTCCTCA	ATCCGCCTGATAGGGAATCA	55.0	50.0	58.1	58.0	678-792 bp	0.964286	89.9
ycf1_p2	CCCTTGTCTCTTCCTCCTCA	TCCGCCTGATAGGGAATCAT	55.0	50.0	58.1	58.0	677-791 bp	0.964286	89.9
ycf1_p3	CCCTTGTCTCTTCCTCCTCA	AGAATCCGCCTGATAGGGAA	55.0	50.0	58.1	57.9	681-795 bp	0.886905	86.2
ycf1_p4	TCTCTCCCCACCAAATTCCT	ATCCGCCTGATAGGGAATCA	50.0	50.0	57.9	58.0	658-667 bp	0.761905	81.1
ycf1_p5	TCTCTCCCCACCAAATTCCT	TCCGCCTGATAGGGAATCAT	50.0	50.0	57.9	58.0	657-666 bp	0.761905	81.1