Hotspot

ccsA

SSC · 1881-2834 · 954 bp

Back to Tillandsia

Pi 0.00565

MarkerSeek score 51.80

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 954
variable_sites: 71
pi: 0.00565
unique_haplotype_count: 71
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	GCTAAAGCATCTCGGTTCGA	CTTCATGTAGTTTATTCAATTAGATGT	50.0	25.9	58.1	54.4	1081-1096 bp	0.964286	48.0
ccsA_p2	AGTGGCGGCATCTTCTAAAA	CTTCATGTAGTTTATTCAATTAGATGT	45.0	25.9	57.5	54.4	1056-1066 bp	0.958333	47.9
ccsA_p3	GCTAAAGCATCTCGGTTCGA	TCTTCATGTAGTTTATTCAATTAGATG	50.0	25.9	58.1	54.2	1082-1097 bp	0.958333	47.8
ccsA_p4	GAGTGGCGGCATCTTCTAAA	CTTCATGTAGTTTATTCAATTAGATGT	50.0	25.9	58.0	54.4	1057-1067 bp	0.952381	47.6
ccsA_p5	GAGTGGCGGCATCTTCTAAA	TCTTCATGTAGTTTATTCAATTAGATG	50.0	25.9	58.0	54.2	1058-1068 bp	0.946429	47.4