Hotspot

psbE-petL

LSC · 109173-110696 · 1524 bp

Back to Tillandsia

Pi 0.00962

MarkerSeek score 64.00

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1524
variable_sites: 239
pi: 0.00962
unique_haplotype_count: 133
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAACCCGCAATGAATAGGGA	AAGCAGCCAGTAGAAAACCA	50.0	45.0	57.9	57.3	1256-2942 bp	1.0	86.3
psbE-petL_p2	CAACCCGCAATGAATAGGGA	AGCAGCCAGTAGAAAACCAA	50.0	45.0	57.9	57.3	1255-2941 bp	1.0	86.3
psbE-petL_p3	ACTGACGAATAACCAACCCG	AAGCAGCCAGTAGAAAACCA	50.0	45.0	57.9	57.3	1269-2945 bp	1.0	86.0
psbE-petL_p4	ACTGACGAATAACCAACCCG	AGCAGCCAGTAGAAAACCAA	50.0	45.0	57.9	57.3	1268-2944 bp	1.0	86.0
psbE-petL_p5	ACGAATAACCAACCCGCAAT	AGCAGCCAGTAGAAAACCAA	45.0	45.0	58.2	57.3	1264-2939 bp	1.0	85.4