Hotspot

atpB-rbcL

LSC · 98282-99061 · 780 bp

Back to Tillandsia

Pi 0.00849

MarkerSeek score 68.30

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 780
variable_sites: 81
pi: 0.00849
unique_haplotype_count: 100
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 68.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	GGGGAAAAACGACATCCAGT	GGTGACATAAGTCCCTCCCT	50.0	55.0	58.1	58.1	714-926 bp	1.0	90.3
atpB-rbcL_p2	TGGGCCAATAATTTGAGCGA	GGTGACATAAGTCCCTCCCT	45.0	55.0	57.9	58.1	692-904 bp	0.988095	89.4
atpB-rbcL_p3	GGGGAAAAACGACATCCAGT	TGGTGACATAAGTCCCTCCC	50.0	55.0	58.1	58.4	715-927 bp	1.0	87.9
atpB-rbcL_p4	TGGGCCAATAATTTGAGCGA	TGGTGACATAAGTCCCTCCC	45.0	55.0	57.9	58.4	693-905 bp	0.988095	86.9
atpB-rbcL_p5	CTGGGCCAATAATTTGAGCG	GGTGACATAAGTCCCTCCCT	50.0	55.0	57.5	58.1	693-905 bp	0.988095	86.5