Hotspot

ndhC-trnV-UAC

LSC · 94272-95312 · 1041 bp

Back to Tillandsia

Pi 0.00936

MarkerSeek score 70.90

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 1041
variable_sites: 172
pi: 0.00936
unique_haplotype_count: 124
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	CTAACCGGGGCTAAAACTCC	AGAAGGTCTACGGTTCGAGT	55.0	50.0	58.0	57.8	814-1228 bp	1.0	90.1
ndhC-trnV-UAC_p2	CTAACCGGGGCTAAAACTCC	GAGCCCAATGCAACAAACAA	55.0	45.0	58.0	57.8	901-1315 bp	0.982143	89.1
ndhC-trnV-UAC_p3	CGGGGCTAAAACTCCTGAAA	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.8	57.8	809-1223 bp	1.0	88.9
ndhC-trnV-UAC_p4	CGGGGCTAAAACTCCTGAAA	GAGCCCAATGCAACAAACAA	50.0	45.0	57.8	57.8	896-1310 bp	0.982143	87.8
ndhC-trnV-UAC_p5	TAACCGGGGCTAAAACTCCT	AGAAGGTCTACGGTTCGAGT	50.0	50.0	58.3	57.8	813-1227 bp	1.0	87.7