Hotspot

psaA-ycf3

LSC · 85394-86040 · 647 bp

Back to Tillandsia

Pi 0.0122

MarkerSeek score 67.70

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 647
variable_sites: 71
pi: 0.0122
unique_haplotype_count: 77
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	CCCATTCCTCGAAAGACGTT	GTTGAAAATCACGAAGCGCT	50.0	45.0	57.9	57.7	675-795 bp	1.0	88.7
psaA-ycf3_p2	CCCATTCCTCGAAAGACGTT	GCTATAGCGCTTACTCCAGG	50.0	55.0	57.9	57.6	719-839 bp	0.994048	87.6
psaA-ycf3_p3	CCCATTCCTCGAAAGACGTT	CCGATCAAGCTGCTGAGTAT	50.0	50.0	57.9	57.5	748-868 bp	1.0	86.8
psaA-ycf3_p4	CCCATTCCTCGAAAGACGTT	TCCGATCAAGCTGCTGAGTA	50.0	50.0	57.9	58.5	749-869 bp	1.0	86.7
psaA-ycf3_p5	CCCATTCCTCGAAAGACGTT	TGAAAATCACGAAGCGCTTC	50.0	45.0	57.9	57.4	673-793 bp	1.0	86.5