Hotspot

trnT-GGU-psbD

LSC · 75525-76566 · 1042 bp

Back to Tillandsia

Pi 0.00917

MarkerSeek score 58.50

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1042
variable_sites: 141
pi: 0.00917
unique_haplotype_count: 108
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GCATCGACCCAGGAAGAATT	CACCGTCACCATCTTCGAAT	50.0	50.0	58.0	58.0	1825-2285 bp	0.994048	91.3
trnT-GGU-psbD_p2	GTGGTAGAGTAACGCCATGG	CACCGTCACCATCTTCGAAT	55.0	50.0	58.1	58.0	1368-1816 bp	0.994048	91.2
trnT-GGU-psbD_p3	CATAGTACCCTACCCCCAGG	CACCGTCACCATCTTCGAAT	60.0	50.0	58.0	58.0	1766-2220 bp	0.988095	91.2
trnT-GGU-psbD_p4	TGGAGCGGTGAATTATTGGG	CACCGTCACCATCTTCGAAT	50.0	50.0	57.9	58.0	1915-2375 bp	0.97619	90.6
trnT-GGU-psbD_p5	CATAGTACCCTACCCCCAGG	AAGGAAAGAGCAATAGGCCG	60.0	50.0	58.0	58.0	1199-1653 bp	0.97619	90.5