Hotspot

petN-psbM

LSC · 72053-73289 · 1237 bp

Back to Tillandsia

Pi 0.00858

MarkerSeek score 67.00

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1237
variable_sites: 184
pi: 0.00858
unique_haplotype_count: 117
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	AGTATGGGGAAGGAGTGGAC	TGCTACTGCACTGTTCATTCT	55.0	42.9	58.1	57.6	850-1419 bp	1.0	79.8
petN-psbM_p2	GTATGGGGAAGGAGTGGACT	TGCTACTGCACTGTTCATTCT	55.0	42.9	58.1	57.6	849-1418 bp	1.0	79.8
petN-psbM_p3	TAGTAAGTCTCGCTTGGGCT	TGCTACTGCACTGTTCATTCT	50.0	42.9	58.2	57.6	908-1477 bp	1.0	79.5
petN-psbM_p4	TATGGGGAAGGAGTGGACTC	TGCTACTGCACTGTTCATTCT	55.0	42.9	57.8	57.6	848-1417 bp	1.0	79.3
petN-psbM_p5	GGGGAAGGAGTGGACTCTAG	TGCTACTGCACTGTTCATTCT	60.0	42.9	58.2	57.6	845-1414 bp	0.988095	78.5