Hotspot

trnC-GCA-petN

LSC · 70935-71962 · 1028 bp

Back to Tillandsia

Pi 0.0175
MarkerSeek score 68.50
Primer available yes
Label trnC-GCA-petN

Feature Metadata

label_name
trnC-GCA-petN
feature_type
igs
region
LSC
length_bp
1028
variable_sites
234
pi
0.0175
unique_haplotype_count
134
primer_available
yes
alignment_reliability
1.00
markerseek_score
68.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnC-GCA-petN_p1 AGTTCAAATCTGGGTGTCGC AGTCCACTCCTTCCCCATAC 50.0 55.0 58.5 58.1 699-1158 bp 1.0 87.2
trnC-GCA-petN_p2 AGTTCAAATCTGGGTGTCGC GTCCACTCCTTCCCCATACT 50.0 55.0 58.5 58.1 698-1157 bp 1.0 87.2
trnC-GCA-petN_p3 AGTTCAAATCTGGGTGTCGC AGCCCAAGCGAGACTTACTA 50.0 50.0 58.5 58.2 640-1099 bp 1.0 87.0
trnC-GCA-petN_p4 AGTTCAAATCTGGGTGTCGC GAGTCCACTCCTTCCCCATA 50.0 55.0 58.5 57.8 700-1159 bp 1.0 86.8
trnC-GCA-petN_p5 AGTTCAAATCTGGGTGTCGC CTAGAGTCCACTCCTTCCCC 50.0 60.0 58.5 58.2 703-1162 bp 1.0 86.5