Hotspot

rpl32-trnL-UAG

SSC · 972-1725 · 754 bp

Back to Tillandsia

Pi 0.0186

MarkerSeek score 74.00

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 754
variable_sites: 168
pi: 0.0186
unique_haplotype_count: 110
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	CACCGGGCATTCAAAAAGTT	AGCGTGTCTACCGATTTCAC	45.0	50.0	57.5	58.0	778-904 bp	1.0	87.7
rpl32-trnL-UAG_p2	CACCGGGCATTCAAAAAGTT	CAGCGTGTCTACCGATTTCA	45.0	50.0	57.5	58.0	779-905 bp	1.0	87.7
rpl32-trnL-UAG_p3	CACCGGGCATTCAAAAAGTT	TTTAGAAGATGCCGCCACTC	45.0	50.0	57.5	58.0	838-964 bp	0.994048	87.4
rpl32-trnL-UAG_p4	TTCCACCGGGCATTCAAAAA	AGCGTGTCTACCGATTTCAC	45.0	50.0	58.6	58.0	781-876 bp	0.982143	86.5
rpl32-trnL-UAG_p5	TTTCCACCGGGCATTCAAAA	AGCGTGTCTACCGATTTCAC	45.0	50.0	58.6	58.0	782-877 bp	0.982143	86.5