Pi
0.0052
MarkerSeek score
44.10
Primer available
yes
Label
clpP1
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| clpP1_p1 | TGGATTGGTGTTAAATCGTTGC | ACGCTTCCACATCAAAGTGA | 40.9 | 45.0 | 57.8 | 57.7 | 2251-2305 bp | 1.0 | 63.7 |
| clpP1_p2 | GGATTGGTGTTAAATCGTTGCT | ACGCTTCCACATCAAAGTGA | 40.9 | 45.0 | 57.6 | 57.7 | 2250-2304 bp | 1.0 | 61.5 |
| clpP1_p3 | GGATTGGTGTTAAATCGTTGC | ACGCTTCCACATCAAAGTGA | 42.9 | 45.0 | 56.1 | 57.7 | 2250-2304 bp | 1.0 | 58.1 |
| clpP1_p4 | GGTGTTAAATCGTTGCTATTGCT | ACGCTTCCACATCAAAGTGA | 39.1 | 45.0 | 58.0 | 57.7 | 2245-2299 bp | 1.0 | 56.9 |
| clpP1_p5 | TGTGGATTGGTGTTAAATCGTTG | ACGCTTCCACATCAAAGTGA | 39.1 | 45.0 | 57.9 | 57.7 | 2253-2307 bp | 1.0 | 56.1 |