Hotspot

rps19-rpl2

IRb · 91194-91260 · 67 bp

Back to Tilia

Pi 0.0156
MarkerSeek score 49.00
Primer available yes
Label rps19-rpl2

Feature Metadata

label_name
rps19-rpl2
feature_type
igs
region
IRb
length_bp
67
variable_sites
3
pi
0.0156
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
49.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps19-rpl2_p1 TCCGCTTTTGTATTAAGCCT TTGATTCTTCGTCGCCGTAG 40.0 50.0 55.0 58.1 173-178 bp 1.0 57.4
rps19-rpl2_p2 CGCTTTTGTATTAAGCCTTTCGA TTGATTCTTCGTCGCCGTAG 39.1 50.0 58.0 58.1 171-176 bp 1.0 56.8
rps19-rpl2_p3 TCCGCTTTTGTATTAAGCCT TTGGGGTTATCCTGCACTTG 40.0 50.0 55.0 57.8 231-236 bp 1.0 56.0
rps19-rpl2_p4 TCCGCTTTTGTATTAAGCCTT TTGATTCTTCGTCGCCGTAG 38.1 50.0 55.9 58.1 173-178 bp 1.0 55.9
rps19-rpl2_p5 TTCCGCTTTTGTATTAAGCCT TTGATTCTTCGTCGCCGTAG 38.1 50.0 55.9 58.1 174-179 bp 1.0 55.9