Hotspot

ndhC-trnV-UAC

LSC · 55458-56737 · 1280 bp

Back to Tilia

Pi 0.00424
MarkerSeek score 45.30
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1280
variable_sites
12
pi
0.00424
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.99
markerseek_score
45.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 ACCCTTTCTAATCGGGGCTA AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.8 57.8 1422-1431 bp 1.0 78.2
ndhC-trnV-UAC_p2 CGGGGCTAAAACTCCAGAAA AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.8 57.8 1410-1419 bp 1.0 78.1
ndhC-trnV-UAC_p3 ATCGGGGCTAAAACTCCAGA AGAAGGTCTACGGTTCGAGT 50.0 50.0 58.4 57.8 1412-1421 bp 1.0 76.3
ndhC-trnV-UAC_p4 AATCGGGGCTAAAACTCCAG AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.6 57.8 1413-1422 bp 1.0 76.3
ndhC-trnV-UAC_p5 ACCCTTTCTAATCGGGGCTA GAAGGTCTACGGTTCGAGTC 50.0 55.0 57.8 57.5 1421-1430 bp 1.0 75.4