Hotspot

psbZ-trnG-GCC

LSC · 38594-39521 · 928 bp

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Pi 0.00662
MarkerSeek score 68.70
Primer available yes
Label psbZ-trnG-GCC

Feature Metadata

label_name
psbZ-trnG-GCC
feature_type
igs
region
LSC
length_bp
928
variable_sites
15
pi
0.00662
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.96
markerseek_score
68.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbZ-trnG-GCC_p1 TTGCTTCTCCTGATGGTTGG CGTCTTCTCCTTGGCAAAGA 50.0 50.0 57.8 57.8 1057-1240 bp 1.0 81.6
psbZ-trnG-GCC_p2 GCTTCTCCTGATGGTTGGTT CGTCTTCTCCTTGGCAAAGA 50.0 50.0 57.8 57.8 1055-1238 bp 1.0 81.6
psbZ-trnG-GCC_p3 CTCCTGATGGTTGGTTGAGT CGTCTTCTCCTTGGCAAAGA 50.0 50.0 57.1 57.8 1051-1234 bp 1.0 76.4
psbZ-trnG-GCC_p4 TGCTTCTCCTGATGGTTGGT CGTCTTCTCCTTGGCAAAGA 50.0 50.0 58.9 57.8 1056-1239 bp 1.0 75.8
psbZ-trnG-GCC_p5 TCTCCTGATGGTTGGTTGAG CGTCTTCTCCTTGGCAAAGA 50.0 50.0 56.8 57.8 1052-1235 bp 1.0 74.0