Hotspot

trnT-GGU-psbD

LSC · 33781-35281 · 1501 bp

Back to Tilia

Pi 0.00555
MarkerSeek score 57.50
Primer available yes
Label trnT-GGU-psbD

Feature Metadata

label_name
trnT-GGU-psbD
feature_type
igs
region
LSC
length_bp
1501
variable_sites
19
pi
0.00555
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
57.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-psbD_p1 GTGGTAGAGTAACGCCATGG GAAACGGTCCCTACGTAACC 55.0 55.0 58.1 58.0 1643-1644 bp 1.0 80.4
trnT-GGU-psbD_p2 TCAGTGGTAGAGTAACGCCA GAAACGGTCCCTACGTAACC 50.0 55.0 58.1 58.0 1646-1647 bp 1.0 80.2
trnT-GGU-psbD_p3 GGCGTAAGTCATCGGTTCAA GAAACGGTCCCTACGTAACC 50.0 55.0 58.3 58.0 1620-1621 bp 1.0 78.6
trnT-GGU-psbD_p4 TGGCCCTTTTAACTCAGTGG GAAACGGTCCCTACGTAACC 50.0 55.0 57.7 58.0 1659-1660 bp 1.0 78.6
trnT-GGU-psbD_p5 GGCCCTTTTAACTCAGTGGT GAAACGGTCCCTACGTAACC 50.0 55.0 57.7 58.0 1658-1659 bp 1.0 78.6