Hotspot

rpoB-trnC-GCA

LSC · 28194-29454 · 1261 bp

Back to Tilia

Pi 0.0031
MarkerSeek score 49.20
Primer available yes
Label rpoB-trnC-GCA

Feature Metadata

label_name
rpoB-trnC-GCA
feature_type
igs
region
LSC
length_bp
1261
variable_sites
10
pi
0.0031
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.99
markerseek_score
49.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoB-trnC-GCA_p1 CCCGATACATGAACCCACAA CCGGATTTGAACTGGGGAAT 50.0 50.0 57.6 57.9 1399-1416 bp 1.0 77.1
rpoB-trnC-GCA_p2 GTTCTTCTGTTAAGCCCCGA CCGGATTTGAACTGGGGAAT 50.0 50.0 57.5 57.9 1414-1431 bp 1.0 76.6
rpoB-trnC-GCA_p3 AGTTCTTCTGTTAAGCCCCG CCGGATTTGAACTGGGGAAT 50.0 50.0 57.5 57.9 1415-1432 bp 1.0 76.6
rpoB-trnC-GCA_p4 ATTTCCCCATTTGCATCCCC CCGGATTTGAACTGGGGAAT 50.0 50.0 58.5 57.9 1337-1354 bp 1.0 76.5
rpoB-trnC-GCA_p5 CATTTCCCCATTTGCATCCC CCGGATTTGAACTGGGGAAT 50.0 50.0 57.4 57.9 1338-1355 bp 1.0 75.4