Hotspot

atpF-atpH

LSC · 13759-14380 · 622 bp

Back to Tilia

Pi 0.00413
MarkerSeek score 48.90
Primer available yes
Label atpF-atpH

Feature Metadata

label_name
atpF-atpH
feature_type
igs
region
LSC
length_bp
622
variable_sites
6
pi
0.00413
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
48.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpF-atpH_p1 CAGTGACCCAAGGAAACGAA TGGTTGTAGCATTGGCACTT 50.0 45.0 58.0 58.0 706-712 bp 1.0 80.7
atpF-atpH_p2 ATTAAACCCGAAACTCCCGG TGGTTGTAGCATTGGCACTT 50.0 45.0 57.9 58.0 734-740 bp 1.0 80.0
atpF-atpH_p3 CAGTGACCCAAGGAAACGAA CTGGTTGTAGCATTGGCACT 50.0 50.0 58.0 58.5 707-713 bp 1.0 77.0
atpF-atpH_p4 CAGTGACCCAAGGAAACGAA ATGGGCTGGTTGTAGCATTG 50.0 50.0 58.0 58.5 712-718 bp 1.0 76.5
atpF-atpH_p5 ATTAAACCCGAAACTCCCGG CTGGTTGTAGCATTGGCACT 50.0 50.0 57.9 58.5 735-741 bp 1.0 76.3