Hotspot

rps16-trnQ-UUG

LSC · 6715-7250 · 536 bp

Back to Tilia

Pi 0.00711
MarkerSeek score 42.70
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
536
variable_sites
11
pi
0.00711
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.98
markerseek_score
42.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 AGTGGTGGGTTTTGACATCC GAAATCAAAATGGGGCGTGG 50.0 50.0 58.0 58.0 706-711 bp 1.0 82.3
rps16-trnQ-UUG_p2 CAGCCGATCATGTCCTTCAA GAAATCAAAATGGGGCGTGG 50.0 50.0 58.0 58.0 685-690 bp 1.0 82.1
rps16-trnQ-UUG_p3 GCCGATCATGTCCTTCAAGT GAAATCAAAATGGGGCGTGG 50.0 50.0 58.0 58.0 683-688 bp 1.0 82.1
rps16-trnQ-UUG_p4 AGTGGTGGGTTTTGACATCC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 648-653 bp 1.0 81.9
rps16-trnQ-UUG_p5 CAGCCGATCATGTCCTTCAA GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 627-632 bp 1.0 81.8