Hotspot

trnK-UUU-rps16

LSC · 4692-5588 · 897 bp

Back to Tilia

Pi 0.00554
MarkerSeek score 50.40
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
897
variable_sites
10
pi
0.00554
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
50.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AAAGCCGAGTACTCTACCGT AAAAGGAGCTCAACCCACAG 50.0 50.0 58.2 58.0 1023-1058 bp 1.0 80.5
trnK-UUU-rps16_p2 AAGCCGAGTACTCTACCGTT AAAAGGAGCTCAACCCACAG 50.0 50.0 58.2 58.0 1022-1057 bp 1.0 80.5
trnK-UUU-rps16_p3 TACCGTTGAGTTAGCAACCC AAAAGGAGCTCAACCCACAG 50.0 50.0 57.5 58.0 1009-1044 bp 1.0 78.2
trnK-UUU-rps16_p4 AAAGCCGAGTACTCTACCGT AAGAAGGCGGGGGTTTTTAC 50.0 50.0 58.2 58.4 983-1018 bp 1.0 77.6
trnK-UUU-rps16_p5 AAGCCGAGTACTCTACCGTT AAGAAGGCGGGGGTTTTTAC 50.0 50.0 58.2 58.4 982-1017 bp 1.0 77.6