Hotspot

trnH-GUG-psbA

LSC · 133-744 · 612 bp

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Pi 0.0168
MarkerSeek score 65.60
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
612
variable_sites
29
pi
0.0168
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.98
markerseek_score
65.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 ATTAGACATGGGCGAACGAC TTCCCTCTAGACCTAGCTGC 50.0 55.0 58.1 57.9 705-814 bp 1.0 85.9
trnH-GUG-psbA_p2 GATCCACTTGGCTACATCCG TTCCCTCTAGACCTAGCTGC 55.0 55.0 58.1 57.9 642-751 bp 1.0 85.6
trnH-GUG-psbA_p3 CAATCCACTGCCTTGATCCA TTCCCTCTAGACCTAGCTGC 50.0 55.0 57.9 57.9 656-765 bp 1.0 85.5
trnH-GUG-psbA_p4 AATCCACTGCCTTGATCCAC TTCCCTCTAGACCTAGCTGC 50.0 55.0 57.9 57.9 655-764 bp 1.0 85.5
trnH-GUG-psbA_p5 ACAATCCACTGCCTTGATCC TTCCCTCTAGACCTAGCTGC 50.0 55.0 57.9 57.9 657-766 bp 1.0 85.5