Hotspot

petD

LSC · 77566-78857 · 1292 bp

Back to Tigridiopalma

Pi 0.00413

MarkerSeek score 61.50

Primer available yes

Label petD

Feature Metadata

label_name: petD
feature_type: gene
region: LSC
length_bp: 1292
variable_sites: 8
pi: 0.00413
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD_p1	TGAGGGGAATTCTACGACGA	GCATAAGCCGCGCTATTCTA	50.0	50.0	57.9	58.3	1365-1366 bp	1.0	77.1
petD_p2	TCCCTTCACTAGTCGTGTCA	GCATAAGCCGCGCTATTCTA	50.0	50.0	57.7	58.3	1410-1411 bp	1.0	75.9
petD_p3	TGAGGGGAATTCTACGACGA	TTCCCTAGATGCATAAGCCG	50.0	50.0	57.9	57.2	1375-1376 bp	1.0	72.7
petD_p4	TCCCTTCACTAGTCGTGTCA	TTCCCTAGATGCATAAGCCG	50.0	50.0	57.7	57.2	1420-1421 bp	1.0	71.5
petD_p5	TGAGGGGAATTCTACGACGA	ATGCATAAGCCGCGCTATT	50.0	47.4	57.9	58.1	1367-1368 bp	1.0	70.9