Hotspot

ndhF

SSC · 112058-114295 · 2238 bp

Back to Tigridiopalma

Pi 0.00626

MarkerSeek score 48.90

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2238
variable_sites: 21
pi: 0.00626
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	TTTGAATGGTCCCTATCGCG	TCGATTGCACTATTAGCCTGA	50.0	42.9	58.1	56.9	2665-2671 bp	1.0	63.5
ndhF_p2	TGTCAACCTTCCGGGAAATG	TCGATTGCACTATTAGCCTGA	50.0	42.9	58.1	56.9	2915-2921 bp	1.0	63.1
ndhF_p3	CCTATCGCGGAAGGAGAAAC	TCGATTGCACTATTAGCCTGA	55.0	42.9	58.1	56.9	2654-2660 bp	1.0	62.7
ndhF_p4	TCGCGGAAGGAGAAACATTT	TCGATTGCACTATTAGCCTGA	45.0	42.9	57.8	56.9	2650-2656 bp	1.0	62.5
ndhF_p5	GTCAACCTTCCGGGAAATGA	TCGATTGCACTATTAGCCTGA	50.0	42.9	57.8	56.9	2914-2920 bp	1.0	62.3