Hotspot

ccsA-ndhD

SSC · 116654-116982 · 329 bp

Back to Tigridiopalma

Pi 0.0344

MarkerSeek score 52.90

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 329
variable_sites: 17
pi: 0.0344
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	CTTCTCTTGTTCCGCGAGAA	TCAATGGCTTCCCTTGCATT	50.0	45.0	57.9	58.1	1059-1060 bp	1.0	81.4
ccsA-ndhD_p2	ATGCAGCTCTTTTATGCGGA	TCAATGGCTTCCCTTGCATT	45.0	45.0	58.0	58.1	1210-1211 bp	1.0	81.3
ccsA-ndhD_p3	TGCAGCTCTTTTATGCGGAT	TCAATGGCTTCCCTTGCATT	45.0	45.0	58.0	58.1	1209-1210 bp	1.0	81.3
ccsA-ndhD_p4	TTAGTACCCGCGCTTCAATC	TCAATGGCTTCCCTTGCATT	50.0	45.0	58.1	58.1	1271-1272 bp	1.0	81.0
ccsA-ndhD_p5	TACACGGCTTTACCACTTCG	TCAATGGCTTCCCTTGCATT	50.0	45.0	57.9	58.1	1324-1325 bp	1.0	80.9