Hotspot

petL-petG

LSC · 66445-66631 · 187 bp

Back to Tigridiopalma

Pi 0.00713

MarkerSeek score 29.90

Primer available yes

Label petL-petG

Feature Metadata

label_name: petL-petG
feature_type: igs
region: LSC
length_bp: 187
variable_sites: 2
pi: 0.00713
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 29.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petL-petG_p1	AGACTCTACTTCCCGTAGTCT	AGCGCTCTTAGTTCAGTTCG	47.6	50.0	56.7	58.0	790-791 bp	1.0	63.3
petL-petG_p2	AGACTCTACTTCCCGTAGTCT	AGAACGTGGGTCTCCAAAAC	47.6	50.0	56.7	58.0	768-769 bp	1.0	63.2
petL-petG_p3	AGACTCTACTTCCCGTAGTCT	CAGAACGTGGGTCTCCAAAA	47.6	50.0	56.7	58.0	769-770 bp	1.0	63.2
petL-petG_p4	AGACTCTACTTCCCGTAGTCT	AAGAAAGGGATGTAGCGCAG	47.6	50.0	56.7	58.0	1060-1061 bp	1.0	62.9
petL-petG_p5	AGACTCTACTTCCCGTAGTCT	GCTCTTAGTTCAGTTCGGCA	47.6	50.0	56.7	57.9	787-788 bp	1.0	62.8