Hotspot

petA-psbJ

LSC · 63519-64395 · 877 bp

Back to Tigridiopalma

Pi 0.0106

MarkerSeek score 63.20

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 877
variable_sites: 13
pi: 0.0106
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	TGTTCTTCCTGGCATCTGTT	ATACTCAGGGTTGGGCTCAT	45.0	50.0	57.0	58.1	912-1000 bp	1.0	76.6
petA-psbJ_p2	TTGTTCTTCCTGGCATCTGT	ATACTCAGGGTTGGGCTCAT	45.0	50.0	57.0	58.1	913-1001 bp	1.0	76.6
petA-psbJ_p3	GGTCTTTTGTTCTTCCTGGC	ATACTCAGGGTTGGGCTCAT	50.0	50.0	56.9	58.1	919-1007 bp	1.0	75.6
petA-psbJ_p4	TGTTCTTCCTGGCATCTGTT	GTTCATACTCAGGGTTGGGC	45.0	55.0	57.0	58.2	916-1004 bp	1.0	75.4
petA-psbJ_p5	TTGTTCTTCCTGGCATCTGT	GTTCATACTCAGGGTTGGGC	45.0	55.0	57.0	58.2	917-1005 bp	1.0	75.4