Hotspot

ndhC-trnV-UAC

LSC · 51532-51985 · 454 bp

Back to Tigridiopalma

Pi 0.0206

MarkerSeek score 67.10

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 454
variable_sites: 14
pi: 0.0206
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	GACCTTTACTAATCGGGGCG	GGTTCGAATCCGTATAGCCC	55.0	55.0	58.1	57.9	587-596 bp	1.0	80.3
ndhC-trnV-UAC_p2	GGACCTTTACTAATCGGGGC	GGTTCGAATCCGTATAGCCC	55.0	55.0	57.7	57.9	588-597 bp	1.0	79.4
ndhC-trnV-UAC_p3	GACCTTTACTAATCGGGGCG	CGGTTCGAATCCGTATAGCC	55.0	55.0	58.1	58.3	588-597 bp	1.0	78.5
ndhC-trnV-UAC_p4	GGACCTTTACTAATCGGGGC	CGGTTCGAATCCGTATAGCC	55.0	55.0	57.7	58.3	589-598 bp	1.0	77.6
ndhC-trnV-UAC_p5	GACCTTTACTAATCGGGGCG	CGAAAGAGCCCAATGCAAAA	55.0	45.0	58.1	57.6	685-694 bp	1.0	77.1