Hotspot

trnD-GUC-trnY-GUA

LSC · 30635-31059 · 425 bp

Back to Tigridiopalma

Pi 0.0152
MarkerSeek score 63.40
Primer available yes
Label trnD-GUC-trnY-GUA

Feature Metadata

label_name
trnD-GUC-trnY-GUA
feature_type
igs
region
LSC
length_bp
425
variable_sites
8
pi
0.0152
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.98
markerseek_score
63.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnD-GUC-trnY-GUA_p1 GCGGTGCTCTAACCAATTGA TGGCAATATGTCTACGCTGG 50.0 50.0 58.6 57.8 482-521 bp 1.0 77.2
trnD-GUC-trnY-GUA_p2 GCGGTGCTCTAACCAATTGA GGCAATATGTCTACGCTGGT 50.0 50.0 58.6 57.8 481-520 bp 1.0 77.2
trnD-GUC-trnY-GUA_p3 GCGGTGCTCTAACCAATTGA GAATGGCTTCTTCCTGGGTC 50.0 55.0 58.6 58.2 541-580 bp 1.0 76.8
trnD-GUC-trnY-GUA_p4 TTTATTCCGTCGGGACTGAC TGGCAATATGTCTACGCTGG 50.0 50.0 57.3 57.8 535-574 bp 1.0 76.0
trnD-GUC-trnY-GUA_p5 TTTATTCCGTCGGGACTGAC GGCAATATGTCTACGCTGGT 50.0 50.0 57.3 57.8 534-573 bp 1.0 76.0