Hotspot

psbM-trnD-GUC

LSC · 29831-30560 · 730 bp

Back to Tigridiopalma

Pi 0.00822

MarkerSeek score 62.00

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 730
variable_sites: 9
pi: 0.00822
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	TCACTTTGACTCACGGTTTT	TCAATTGGTTAGAGCACCGC	40.0	50.0	55.4	58.6	894-898 bp	1.0	56.5
psbM-trnD-GUC_p2	TCACTTTGACTCACGGTTTTT	TCAATTGGTTAGAGCACCGC	38.1	50.0	56.2	58.6	894-898 bp	1.0	54.9
psbM-trnD-GUC_p3	AATCACTTTGACTCACGGTT	TCAATTGGTTAGAGCACCGC	40.0	50.0	55.2	58.6	896-900 bp	1.0	54.6
psbM-trnD-GUC_p4	ATCACTTTGACTCACGGTTT	TCAATTGGTTAGAGCACCGC	40.0	50.0	55.2	58.6	895-899 bp	1.0	54.6
psbM-trnD-GUC_p5	AGAATGAAGAGTGCAGTAGC	TCAATTGGTTAGAGCACCGC	45.0	50.0	55.2	58.6	840-844 bp	1.0	54.5