Hotspot

rps15-ycf1

SSC · 128702-129098 · 397 bp

Back to Tieghemella

Pi 0.00756

MarkerSeek score 58.90

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 397
variable_sites: 3
pi: 0.00756
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	TCAACAGACCCTCGGTTTTC	TGGCAGTCGTTTCAGTATGT	50.0	45.0	57.8	57.2	511 bp	1.0	71.8
rps15-ycf1_p2	CAACAGACCCTCGGTTTTCT	TGGCAGTCGTTTCAGTATGT	50.0	45.0	57.7	57.2	510 bp	1.0	71.8
rps15-ycf1_p3	TCAACAGACCCTCGGTTTTC	TGGCAGTCGTTTCAGTATGTT	50.0	42.9	57.8	57.9	511 bp	1.0	69.5
rps15-ycf1_p4	CAACAGACCCTCGGTTTTCT	TGGCAGTCGTTTCAGTATGTT	50.0	42.9	57.7	57.9	510 bp	1.0	69.5
rps15-ycf1_p5	ACAGACCCTCGGTTTTCTTC	TGGCAGTCGTTTCAGTATGT	50.0	45.0	57.5	57.2	508 bp	1.0	69.5