Hotspot

ndhF-rpl32

SSC · 117311-118376 · 1066 bp

Back to Tieghemella

Pi 0.00096
MarkerSeek score 61.50
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
1066
variable_sites
1
pi
0.00096
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
61.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 AAAAGAAGAAGCCCCACTCC AAAAGCTTTTAAGGCTGCCT 50.0 40.0 57.7 56.1 1224-1248 bp 1.0 64.1
ndhF-rpl32_p2 AAAAGAAGAAGCCCCACTCC AAAGCTTTTAAGGCTGCCTT 50.0 40.0 57.7 56.1 1223-1247 bp 1.0 64.1
ndhF-rpl32_p3 CTGGAAGTGGAACAAACGGT AAAAGCTTTTAAGGCTGCCT 50.0 40.0 58.3 56.1 1190-1214 bp 1.0 63.9
ndhF-rpl32_p4 CTGGAAGTGGAACAAACGGT AAAGCTTTTAAGGCTGCCTT 50.0 40.0 58.3 56.1 1189-1213 bp 1.0 63.9
ndhF-rpl32_p5 AAACGGTATGATCCACGCAT AAAGCTTTTAAGGCTGCCTT 45.0 40.0 57.7 56.1 1176-1200 bp 1.0 63.9