Hotspot

rpl20-rps12

LSC · 73416-74206 · 791 bp

Back to Tieghemella

Pi 0

MarkerSeek score 59.10

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: LSC
length_bp: 791
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	CTTGAATGAGCCCCTCGAAA	AAATGTCACGAAATCCCCCG	50.0	50.0	57.9	58.5	947-950 bp	1.0	75.0
rpl20-rps12_p2	TTGAATGAGCCCCTCGAAAG	AAATGTCACGAAATCCCCCG	50.0	50.0	57.9	58.5	946-949 bp	1.0	75.0
rpl20-rps12_p3	CTTGAATGAGCCCCTCGAAA	CAGCGTCGAGGAACTTGTAC	50.0	55.0	57.9	58.7	910-913 bp	1.0	74.1
rpl20-rps12_p4	TTGAATGAGCCCCTCGAAAG	CAGCGTCGAGGAACTTGTAC	50.0	55.0	57.9	58.7	909-912 bp	1.0	74.1
rpl20-rps12_p5	TCCCCGTCTAATTCTGGTCA	AAATGTCACGAAATCCCCCG	50.0	50.0	57.8	58.5	879-882 bp	1.0	74.0