Hotspot

rps16-trnQ-UUG

LSC · 6475-8502 · 2028 bp

Back to Tieghemella

Pi 0.00296
MarkerSeek score 59.40
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
2028
variable_sites
6
pi
0.00296
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 GTCTTTCAAGTCGCACGTTG GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 2108 bp 1.0 79.6
rps16-trnQ-UUG_p2 TCCTGTCTTTCAAGTCGCAC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.1 58.1 2112 bp 1.0 78.5
rps16-trnQ-UUG_p3 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 2101 bp 1.0 78.5
rps16-trnQ-UUG_p4 CCACAACGGATCCTGTCTTT GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.8 58.1 2122 bp 1.0 78.1
rps16-trnQ-UUG_p5 GTCTTTCAAGTCGCACGTTG AAATTTAAATGGGGCGTGGC 50.0 45.0 58.0 57.6 2165 bp 1.0 77.0