Hotspot

petB

LSC · 75031-76471 · 1441 bp

Back to Tibetiodes

Pi 0.0111

MarkerSeek score 53.40

Primer available yes

Label petB

Feature Metadata

label_name: petB
feature_type: gene
region: LSC
length_bp: 1441
variable_sites: 22
pi: 0.0111
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petB_p1	GTGGAATTTCTTTGTTTCTGT	GATCTATGCTATATCTTCTCTATTCT	33.3	30.8	52.8	53.5	1499 bp	0.333333	13.3
petB_p2	GTGGAATTTCTTTGTTTCTGT	ATCTATGCTATATCTTCTCTATTCT	33.3	28.0	52.8	52.2	1498 bp	0.333333	13.3
petB_p3	GTGGAATTTCTTTGTTTCTGT	GATCTATGCTATATCTTCTCTATTC	33.3	32.0	52.8	52.1	1499 bp	0.333333	13.3
petB_p4	TCTTTGTTTCTGTATTTCTGGA	GATCTATGCTATATCTTCTCTATTCT	31.8	30.8	53.3	53.5	1491 bp	0.333333	13.3
petB_p5	TCTTTGTTTCTGTATTTCTGGAA	GATCTATGCTATATCTTCTCTATTCT	30.4	30.8	54.1	53.5	1491 bp	0.333333	13.3