Hotspot

trnL-UAG-rpl32

SSC · 123707-124491 · 785 bp

Back to Tibetiodes

Pi 0.0184
MarkerSeek score 64.50
Primer available yes
Label trnL-UAG-rpl32

Feature Metadata

label_name
trnL-UAG-rpl32
feature_type
igs
region
SSC
length_bp
785
variable_sites
21
pi
0.0184
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.99
markerseek_score
64.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-UAG-rpl32_p1 AGCGTGTCTACCGATTTCAC GGGCGTCGTTAAAAGCTTTG 50.0 50.0 58.0 58.3 903-957 bp 1.0 80.5
trnL-UAG-rpl32_p2 CAGCGTGTCTACCGATTTCA GGGCGTCGTTAAAAGCTTTG 50.0 50.0 58.0 58.3 904-958 bp 1.0 80.5
trnL-UAG-rpl32_p3 AGCGTGTCTACCGATTTCAC GGCGTCGTTAAAAGCTTTGT 50.0 45.0 58.0 57.6 902-956 bp 1.0 79.9
trnL-UAG-rpl32_p4 CAGCGTGTCTACCGATTTCA GGCGTCGTTAAAAGCTTTGT 50.0 45.0 58.0 57.6 903-957 bp 1.0 79.9
trnL-UAG-rpl32_p5 TCCTAAGAGCAGCGTGTCTA GGGCGTCGTTAAAAGCTTTG 50.0 50.0 57.9 58.3 913-967 bp 1.0 79.6