Hotspot

pafII-cemA

LSC · 60477-61356 · 880 bp

Back to Tibetiodes

Pi 0.0256

MarkerSeek score 72.70

Primer available yes

Label pafII-cemA

Feature Metadata

label_name: pafII-cemA
feature_type: igs
region: LSC
length_bp: 880
variable_sites: 33
pi: 0.0256
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 72.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
pafII-cemA_p1	GAGCCATTCCATTGACTCGT	GAGAAATCCACCAGGGCAAA	50.0	50.0	58.0	58.1	1040-1074 bp	1.0	84.9
pafII-cemA_p2	CTCCACGGGAAATGGAACAA	GAGAAATCCACCAGGGCAAA	50.0	50.0	58.1	58.1	1004-1038 bp	1.0	84.6
pafII-cemA_p3	GAGCCATTCCATTGACTCGT	AGAAATCCACCAGGGCAAAA	50.0	45.0	58.0	57.6	1039-1073 bp	1.0	82.3
pafII-cemA_p4	CTCCACGGGAAATGGAACAA	AGAAATCCACCAGGGCAAAA	50.0	45.0	58.1	57.6	1003-1037 bp	1.0	82.0
pafII-cemA_p5	GGAGCCATTCCATTGACTCG	GAGAAATCCACCAGGGCAAA	55.0	50.0	58.7	58.1	1041-1075 bp	1.0	79.6