Hotspot

rbcL-accD

LSC · 56524-57041 · 518 bp

Back to Tibetiodes

Pi 0.0248

MarkerSeek score 47.20

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 518
variable_sites: 19
pi: 0.0248
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 47.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	GCCGTAGCTAATCGAGTAGC	CTTTCGCCGGCATTTTCAAT	55.0	45.0	58.0	58.0	881-889 bp	1.0	82.8
rbcL-accD_p2	TTGAATGCTACTGCGGGTAC	CTTTCGCCGGCATTTTCAAT	50.0	45.0	58.0	58.0	1412-1420 bp	1.0	81.9
rbcL-accD_p3	CTAACAGGGGGATTCACTGC	CTTTCGCCGGCATTTTCAAT	55.0	45.0	58.0	58.0	1322-1330 bp	1.0	81.7
rbcL-accD_p4	AATGCTACTGCGGGTACATG	CTTTCGCCGGCATTTTCAAT	50.0	45.0	58.0	58.0	1409-1417 bp	1.0	81.7
rbcL-accD_p5	TTGAATGCTACTGCGGGTAC	ATCTTTACTTTCGCCGGCAT	50.0	45.0	58.0	58.0	1419-1427 bp	1.0	81.6