Hotspot

atpB-rbcL

LSC · 54356-55044 · 689 bp

Back to Tibetiodes

Pi 0.0107

MarkerSeek score 62.00

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 689
variable_sites: 11
pi: 0.0107
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	CTGGCGGAAAAGCTACATCT	CACCAGCTTTGAATCCAACAC	50.0	47.6	58.0	58.0	855-857 bp	1.0	72.8
atpB-rbcL_p2	CTGGCGGAAAAGCTACATCT	ACCAGCTTTGAATCCAACACT	50.0	42.9	58.0	58.1	854-856 bp	1.0	72.6
atpB-rbcL_p3	CTGGCGGAAAAGCTACATCT	GTGGTGACATAAATCCCTCCC	50.0	52.4	58.0	58.1	816-818 bp	1.0	72.5
atpB-rbcL_p4	CTGGCGGAAAAGCTACATCT	TGGTGACATAAATCCCTCCCT	50.0	47.6	58.0	58.1	815-817 bp	1.0	71.9
atpB-rbcL_p5	TTCTTTTTGTCAAGCGTGGC	CACCAGCTTTGAATCCAACAC	45.0	47.6	57.8	58.0	799-801 bp	1.0	71.5