Hotspot

pafI-trnS-GGA

LSC · 44237-45096 · 860 bp

Back to Tibetiodes

Pi 0.00951

MarkerSeek score 62.40

Primer available yes

Label pafI-trnS-GGA

Feature Metadata

label_name: pafI-trnS-GGA
feature_type: igs
region: LSC
length_bp: 860
variable_sites: 12
pi: 0.00951
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 62.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
pafI-trnS-GGA_p1	TTCTCCTGAAGTTGTCGGAA	GGATTCGAACCCTCGGTAAG	45.0	55.0	56.4	57.8	1014-1034 bp	1.0	68.0
pafI-trnS-GGA_p2	TTTCTCCTGAAGTTGTCGGA	GGATTCGAACCCTCGGTAAG	45.0	55.0	56.4	57.8	1015-1035 bp	1.0	68.0
pafI-trnS-GGA_p3	TTCTCCTGAAGTTGTCGGAA	CCTCGGTAAGCAAAAGCCTA	45.0	50.0	56.4	57.6	1004-1024 bp	1.0	66.6
pafI-trnS-GGA_p4	TTTCTCCTGAAGTTGTCGGA	CCTCGGTAAGCAAAAGCCTA	45.0	50.0	56.4	57.6	1005-1025 bp	1.0	66.6
pafI-trnS-GGA_p5	TTCTCCTGAAGTTGTCGGAA	GATTCGAACCCTCGGTAAGC	45.0	55.0	56.4	58.4	1013-1033 bp	1.0	66.3