Hotspot

psbC-trnS-GCU

LSC · 34911-35137 · 227 bp

Back to Tibetiodes

Pi 0.018

MarkerSeek score 64.40

Primer available yes

Label psbC-trnS-GCU

Feature Metadata

label_name: psbC-trnS-GCU
feature_type: igs
region: LSC
length_bp: 227
variable_sites: 6
pi: 0.018
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 64.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC-trnS-GCU_p1	TCTTCGTAGGGCATTTGTGG	AAGTCCCGGTCTTGAAAACC	50.0	50.0	57.9	58.0	399-400 bp	1.0	81.2
psbC-trnS-GCU_p2	CTGTTCTTTCCATGACCCCC	GAGAGATGGCTGAGTGGTTG	55.0	55.0	58.2	58.0	336-337 bp	1.0	81.2
psbC-trnS-GCU_p3	TCTTCGTAGGGCATTTGTGG	GAGAGATGGCTGAGTGGTTG	50.0	55.0	57.9	58.0	420-421 bp	1.0	81.1
psbC-trnS-GCU_p4	TCTTCGTAGGGCATTTGTGG	TGGCTGAGTGGTTGAAAGTC	50.0	50.0	57.9	58.0	414-415 bp	1.0	81.0
psbC-trnS-GCU_p5	TCTTCGTAGGGCATTTGTGG	GTCTTTCGGAGAGATGGCTG	50.0	55.0	57.9	58.1	428-429 bp	1.0	80.7