Hotspot

ycf1

Genome · 104766-109859 · 5094 bp

Back to Tibetia

Pi 0.00471

MarkerSeek score 28.60

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: Genome
length_bp: 5094
variable_sites: 36
pi: 0.00471
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 28.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GAGCTACTGAGGAACAACGG	CGGCTACTTTCTTCTCGGTC	55.0	55.0	58.0	58.1	675-761 bp	1.0	82.9
ycf1_p2	GAGCTACTGAGGAACAACGG	CCAGTAGTTGCGGCTACTTT	55.0	50.0	58.0	57.9	685-771 bp	1.0	82.8
ycf1_p3	GAGCTACTGAGGAACAACGG	TTGGCAAAAGAAGGGGGTTT	55.0	45.0	58.0	58.1	954-1040 bp	1.0	82.0
ycf1_p4	ATCCCGAACCTACACCTAGG	CGGCTACTTTCTTCTCGGTC	55.0	55.0	57.9	58.1	882-968 bp	1.0	81.5
ycf1_p5	ATCCCGAACCTACACCTAGG	CCAGTAGTTGCGGCTACTTT	55.0	50.0	57.9	57.9	892-978 bp	1.0	81.4