Hotspot

trnL-UAG-rpl32

Genome · 119738-120395 · 658 bp

Back to Tibetia

Pi 0.00405
MarkerSeek score 53.50
Primer available yes
Label trnL-UAG-rpl32

Feature Metadata

label_name
trnL-UAG-rpl32
feature_type
igs
region
Genome
length_bp
658
variable_sites
4
pi
0.00405
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
53.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-UAG-rpl32_p1 TCCTAAGAGCAGCGTGTCTA TGGACAGCATTGAAAGCCTT 50.0 45.0 57.9 58.0 750-775 bp 1.0 82.2
trnL-UAG-rpl32_p2 TCCTAAGAGCAGCGTGTCTA TTGGACAGCATTGAAAGCCT 50.0 45.0 57.9 58.0 751-776 bp 1.0 82.2
trnL-UAG-rpl32_p3 AGCAGCGTGTCTACCAATTT TGGACAGCATTGAAAGCCTT 45.0 45.0 57.8 58.0 743-768 bp 1.0 81.8
trnL-UAG-rpl32_p4 AGCAGCGTGTCTACCAATTT TTGGACAGCATTGAAAGCCT 45.0 45.0 57.8 58.0 744-769 bp 1.0 81.8
trnL-UAG-rpl32_p5 CTAAGAGCAGCGTGTCTACC TGGACAGCATTGAAAGCCTT 55.0 45.0 57.8 58.0 748-773 bp 1.0 81.7