Hotspot

rpl14-rpl16

Genome · 76940-77058 · 119 bp

Back to Tibetia

Pi 0.0224

MarkerSeek score 55.20

Primer available yes

Label rpl14-rpl16

Feature Metadata

label_name: rpl14-rpl16
feature_type: igs
region: Genome
length_bp: 119
variable_sites: 4
pi: 0.0224
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl14-rpl16_p1	ACCGTCGATTACTAGCTCCT	AGCAGCATCCAAAATGCCTA	50.0	45.0	57.7	57.9	261-262 bp	1.0	77.6
rpl14-rpl16_p2	ACCGTCGATTACTAGCTCCT	TAGCAGCATCCAAAATGCCT	50.0	45.0	57.7	57.9	262-263 bp	1.0	77.6
rpl14-rpl16_p3	ACCGTCGATTACTAGCTCCT	GGGGTCCCGGAAAATATAGC	50.0	55.0	57.7	57.8	299-300 bp	1.0	77.0
rpl14-rpl16_p4	ACCGTCGATTACTAGCTCCT	ACTTTATGAAATGGGCGGGG	50.0	50.0	57.7	58.2	315-316 bp	1.0	76.7
rpl14-rpl16_p5	ACCGTCGATTACTAGCTCCT	CTTTATGAAATGGGCGGGGT	50.0	50.0	57.7	58.2	314-315 bp	1.0	76.7